Cost-Effective Engineering of a Small-Scale Bioreactor

Authors

John Tansey, Otterbein UniversityFollow
Sadie R. Bartholomew

Document Type

Article

Publication Date

2-1-2007

Publication Title

Cost-Effective Engineering of a Small-Scale Bioreactor

Publisher

Biotechnology and BioEngineering

Keywords

Dissolved oxygen, dissolved oxygen monitoring, CHO cells, Bioreactor, Microcarriers

Abstract

Several methods exist for increasing the scale of cell culture in the laboratory. While these methods provide significant increases in biomass, they are often prohibitively expensive for many laboratories. We have engineered a small-scale bioreactor with a novel means of introducing oxygen through the catalytic breakdown of hydrogen peroxide using a manganese oxide catalyst. We have also adapted and modified an existing assay for dissolved oxygen to be compatible with culture conditions. In this system we have been able to culture CHO cells at densities of up to 10⁷ cells/mL without the use of automated feedback systems.

First Page

401

Last Page

407

Volume

96

Issue

2

Repository Citation

Tansey, John and Bartholomew, Sadie R., "Cost-Effective Engineering of a Small-Scale Bioreactor" (2007). Chemistry Faculty Scholarship. 2.
https://digitalcommons.otterbein.edu/chem_fac/2

Original Citation

Published online 18 August 2006 in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/bit.21118

DOI

10.1002/bit.21118

Version

Post-Print

Peer Reviewed

1