Undergraduate Honors Thesis Projects
Date of Award
Spring 4-2020
Document Type
Honors Paper
Degree Name
Biochemistry and Molecular Biology-BS
Department
Biochemistry and Molecular Biology
Advisor
Simon Lawrance Ph.D.
First Committee Member
Simon Lawrance Ph.D.
Second Committee Member
Sarah Bouchard Ph.D.
Third Committee Member
Prof. Erica Van Dop
Keywords
African Penguin Population, Genetic Diversity, DNA, Denaturing Gradient Gel Electrophoresis, Polymerase Chain Reaction, Allele
Subject Categories
Higher Education | Molecular Biology | Molecular Genetics | Other Ecology and Evolutionary Biology
Abstract
The Major Histocompatibility Complex (MHC) is responsible for the immune response in all jawed vertebrates and protects individuals against a variety of pathogens and diseases. Maintaining genetic diversity within the MHC exons is critical to protecting endangered species. African penguins (Spheniscus demersus) are in danger of losing their MHC diversity in isolated populations due to reductions in population size associated with environmental changes and human activity. This study analyzes the diversity within the exons in the DNA encoding the MHC by amplifying the exons through polymerase chain reaction and identifying alleles through denaturing gradient gel electrophoresis. Wild populations of African penguins from Dassen Island, Dyer Island, and Robben Island in South Africa were sampled. Four alleles were present in the populations analyzed, but the frequencies differed between each island. Cape001 was present at high frequencies within each island population. The Robben Island population had the highest frequency of cape001 compared to the other island populations, with 78.9% of individuals carrying cape001. Analyzing the variability of the MHC II region contributes to previous studies done on populations of African penguins and provides insight into how to further protect the genetic diversity of this endangered species.
Recommended Citation
Schalk, Athena, "Diversity of the Major Histocompatibility Complex in African Penguins (Spheniscus demersus) in situ" (2020). Undergraduate Honors Thesis Projects. 107.
https://digitalcommons.otterbein.edu/stu_honor/107
Included in
Higher Education Commons, Molecular Biology Commons, Molecular Genetics Commons, Other Ecology and Evolutionary Biology Commons