Biology and Earth Science Faculty Scholarship
Document Type
Article
Publication Date
10-2-2011
Publication Title
Biochimica et Biophysica Acta 1821
Publisher
Elsevier
Keywords
Perilipin, Lipolysis, Lipid, trafficking, Lipid droplets, Muscle, Adipose
Abstract
The PAT family of lipid storage droplet proteins comprised five members, each of which has become an established regulator of cellular neutral lipid metabolism. Perilipin 5 (also known as lsdp-5, MLDP, PAT-1, and OXPAT), the most recently discovered member of the family, has been shown to localize to two distinct intracellular pools: the lipid storage droplet (LD), and a poorly characterized cytosolic fraction. We have characterized the denser of these intracellular pools and find that a population of perilipin 5 not associated with large LDs resides in complexes with a discrete density (~1.15 g/ml) and size (~575 kDa). Using immunofluorescence, western blotting of isolated sucrose density fractions, native gradient gel electrophoresis, and co-immunoprecipitation, we have shown that these small (~15 nm), perilipin 5-encoated structures do not contain the PAT protein perilipin 2 (ADRP), but do contain perilipin 3 and several other as of yet uncharacterized proteins. The size and density of these particles as well as their susceptibility to degradation by lipases suggest that like larger LDs, they have a neutral lipid rich core. When treated with oleic acid to promote neutral lipid deposition, cells ectopically expressing perilipin 5 experienced a reorganization of LDs in the cell, resulting in fewer, larger droplets at the expense of smaller ones. Collectively, these data demonstrate that a portion of cytosolic perilipin 5 resides in high density lipid droplet complexes that participate in cellular neutral lipid accumulation.
Last Page
268
Volume
278
Repository Citation
Tansey, John, "Distinct cellular pools of perilipin 5 point to roles in lipid trafficking" (2011). Biology and Earth Science Faculty Scholarship. 21.
https://digitalcommons.otterbein.edu/bio_fac/21
DOI
10.1016/j.bbalip.2011.10.017
Version
Publisher's Version
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.