Date of Award
Biology & Earth Science
Dr. David Sheridan
First Committee Member
Dr. Sarah Bouchard
Second Committee Member
Dr. John Tansey
Isotropic Fractionator, Gliogenesis, Neurogenesis
Biology | Cell and Developmental Biology
During brain development, cells can proliferate and differentiate into either neuronal or glial cells. Neuronal cells are capable of sending signals, while glial cells function to provide nutrients and structural support. The ratio of these cell types can give insight into brain function in comparative studies among species and in studies of phylogenesis and pathology. In order to complete this project in neuroscience, a new technique called the isotropic fractionator was utilized to estimate a neuronal vs. non-neuronal cell count of the rodent brain. Brains ranging from age postnatal day 1 to postnatal day 23 were dissected into 4 regions for analysis: cerebellum, cerebral cortex, midbrain, with specific focus on the olfactory bulb. Nuclei from each region were isolated and stained for analysis using fluorescent microscopy. Peak increase in cell counts began postnatal day 5 and continued until postnatal day 17. Results indicate each region contains a higher proportion of neuronal cells compared to non-neuronal cells.
Maxson, Sarah, "Cell Differentiation and Proliferation During Brain Development" (2019). Honors Thesis Projects. 79.
Available for download on Sunday, April 09, 2023