Undergraduate Honors Thesis Projects

Date of Award

Spring 3-2018

Document Type

Honors Paper

Degree Name

Equine Pre-Veterinary/Pre-Graduate Studies-BS


Equine Science


Dr. Steffanie Burk

First Committee Member

Dr. Jeffrey Lehman

Second Committee Member

Erica Van Dop


cyathostome, papain, benzyl isothiocyanate, papaya, strongyle, equine

Subject Categories

Large or Food Animal and Equine Medicine | Veterinary Pathology and Pathobiology


Cyathostomes are widespread and can affect horses of any life stage. Many common anthelmintics are no longer effective at treating cyathostomes due to parasitic resistance. The objective of this in vitrostudy was to use egg hatch and larval migration tests to determine if compounds found in papaya seeds (papain or benzyl isothiocyanate) reduce cyathostome egg hatching and migration. The experiments were conducted between August 2016 and March 2018. Cyathostome eggs were obtained from horse fecal samples. Egg hatch tests were performed by adding papain and benzyl isothiocyanate (BITC) to wells separately at different concentrations in triplicate. After incubation, the number of unhatched eggs and first stage larvae were counted. Larval migration was performed by incubating fecal samples for 14 days and collecting third stage larvae. The larvae were incubated for two hours at room temperature and then allowed to migrate through mesh for 45 minutes. Papain and BITC were added to wells separately at different concentrations. Each treatment was repeated in triplicate. Values for egg hatch and larval migration were expressed as a percentage of those in the control wells. One-way completely randomized ANOVA was used to compare egg hatch and larval migration among treatments and controls. Overall F-statistics for all experiments indicated differences between concentrations of each compound except papain egg hatch (p