Date of Award
Biochemistry and Molecular Biology-BS
Biology & Earth Science
Dr. Jennifer Bennett
First Committee Member
Dr. Jennifer Bennett
Second Committee Member
Dr. John Tansey
Third Committee Member
Dr. Jonathan DeCoster
cyclic-di-GMP, streptomyces, streptomyces coelicolor, gene expression, RNA sequencing
Bacteriology | Biology | Life Sciences
The second messenger molecule cyclic dimeric GMP (c-di-GMP) regulates a myriad of processes in gram-negative bacteria. However, less is known about the roles it plays in gram-positive bacteria. Differential gene expression in three strains of the gram-positive bacterium, Streptomyces coelicolor, is being studied using RNA sequencing and Real Time PCR to gain insight into c-di-GMP signaling. The rmdA rmdB (regulator of morphology and development) double mutant was compared to the wild type strain known as MT1110. The rmdA and rmdB genes are needed for the formation of aerial mycelium, an important step in the life cycle of Streptomyces coelicolor (Hull et al., 2012). A diguanylate cyclase mutant was also compared to the wild type strain of S. coelicolor. The RNA sequencing experiment revealed that 3,151 genes are differentially expressed in the phosphodiesterase double mutant and 1,341 genes are differentially expressed in the diguanylate cyclase mutant when both mutants are compared to the wild type strain of S. coelicolor. Both known and uncharacterized genes were found in the data to be differentially expressed. Genes of interest for future study were also identified. In particular, the chaplin genes were found to be expressed at significantly lower levels in the phosphodiesterase double mutant as predicted prior to completing the experiment. The chaplins are critical protein players in the formation of aerial hyphae. Real Time PCR experiments are currently being conducted to confirm the results obtained using RNA sequencing.
Stark, Morgan A., "Determination of Cyclic-di-GMP- Controlled Gene Expression in a Pharmacologically Important Bacterium" (2016). Honors Thesis Projects. 30.