Date of Award
Biology & Earth Science
Dr. Jennifer Bennett
First Committee Member
Dr. Jennifer Bennett
Second Committee Member
Dr. David Sheridan
Third Committee Member
Dr. Anthony DeStefanis
Streptomyces, Bacterial Development, Chromosome Segregation, Bacteria
Biology | Cell and Developmental Biology | Higher Education | Molecular Biology
Streptomyces coelicolor is a gram-positive bacterium found in soil. The bacterium can be used for the creation of antibiotics and as a model system for prokaryotic development. Unlike some bacteria, S. coelicolor has linear chromosomes with a multicellular life cycle, consisting of filaments with multi-genomic compartments and later single genomes within spore compartments. During sporulation, chromosome segregation is most noticeable in S. coelicolor along with cellular division to form evenly spaced septa. For chromosomal segregation to take place, the ParABS system is used. ParA and ParB proteins allow for the positioning of the oriC locus during chromosomal segregation. While these proteins control the subcellular localization of oriC, ParA and ParB also organize in the subcellular space to create segregation. While ParAB may play a major role in chromosomal segregation, mutations of each gene only display small phenotypic effects on growth. In addition, chromosomal segregation is strongly correlated with cell division during sporulation, meaning that there is a controlled mechanism for segregation although the exact mechanism remains unknown. The purpose of this study was to characterize a novel gene which may be a contributor to chromosomal segregation in S. coelicolor. This will be done by constructing a deletion mutant of the gene in S. coelicolor and then compared to similar mutants in S. venezuelae.
Copyright, all rights reserved. Fair Use
Brickey, Olivia, "Characterization of Streptomyces coelicolor Transposon Mutants and Deletion of Possible Chromosome Segregation Gene" (2023). Undergraduate Honors Thesis Projects. 154.
Available for download on Sunday, April 18, 2027