Date of Award

Spring 4-10-2025

Document Type

Distinction Paper

Degree Name

Biochemistry and Molecular Biology-BS

Department

Biology & Earth Science

Advisor

Dr. Jennifer Bennett

First Committee Member

Dr. Jennifer Bennett

Second Committee Member

Dr. Carrigan Hayes

Third Committee Member

Dr. Leesa Kern

Keywords

Streptomyces coelicolor, RmdA, RmdB, Cyclic-di-GMP signaling, Antibiotics

Subject Categories

Higher Education | Microbiology

Abstract

Streptomyces coelicolor is a soil bacterium known for its antibiotic production. It utilizes cyclic-di-GMP (c-di-GMP) signaling to regulate important cellular processes like cell cycle progression and antibiotic production. Our research focuses on the specific roles of two key proteins, RmdA and RmdB, in managing c-di-GMP levels, and aims to uncover the specific roles and functions of these proteins. Preliminary data from our lab suggest that RmdB may form dimers, which is important for understanding how c-di-GMP signaling is regulated in Streptomyces coelicolor.

To further investigate, our study uses various methods to examine the impact of RmdA and RmdB on bacterial growth and behavior. These include using different media plates to assess how each protein affects bacterial growth, antibiotic resistance assays to uncover possible differences in sensitivity, and interspecies plating to analyze how Streptomyces coelicolor interacts with other bacterial species. By comparing the effects of RmdA and RmdB under these experimental conditions, we aim to deepen our understanding of how these proteins regulate c-di-GMP signaling and their individual contributions to bacterial processes.

This research could provide valuable insights into bacterial signaling mechanisms and contribute to our understanding of how c-di-GMP influences processes like antibiotic production in Streptomyces. Additionally, it may offer new perspectives on how these proteins can be targeted in future medical applications, such as developing novel antibiotics or controlling bacterial behavior.

Licensing Permission

Copyright, all rights reserved. Fair Use

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Acknowledgement 2

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