Chemistry Faculty Scholarship
Document Type
Article
Publication Date
2-1-2007
Publication Title
Cost-Effective Engineering of a Small-Scale Bioreactor
Publisher
Biotechnology and BioEngineering
Keywords
Dissolved oxygen, dissolved oxygen monitoring, CHO cells, Bioreactor, Microcarriers
Abstract
Several methods exist for increasing the scale of cell culture in the laboratory. While these methods provide significant increases in biomass, they are often prohibitively expensive for many laboratories. We have engineered a small-scale bioreactor with a novel means of introducing oxygen through the catalytic breakdown of hydrogen peroxide using a manganese oxide catalyst. We have also adapted and modified an existing assay for dissolved oxygen to be compatible with culture conditions. In this system we have been able to culture CHO cells at densities of up to 107 cells/mL without the use of automated feedback systems.
First Page
401
Last Page
407
Volume
96
Issue
2
Repository Citation
Tansey, John and Bartholomew, Sadie R., "Cost-Effective Engineering of a Small-Scale Bioreactor" (2007). Chemistry Faculty Scholarship. 2.
https://digitalcommons.otterbein.edu/chem_fac/2
Original Citation
Published online 18 August 2006 in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/bit.21118
DOI
10.1002/bit.21118
Version
Post-Print
Peer Reviewed
1